Pd+2+DNA+Fingerprinting

= Who Invented It? = = DNA fingerprinting was discovered in 1985 by a scientist named Sir Alec Jefferys. = = =

= What does DNA fingerprinting use? = = DNA fingerprinting uses a specific type of DNA sequence, known as a microsatellite, to make identifying something much easier. Microsatellites are short pieces of DNA which repeat many times in a given person's DNA. In any given area, microsatilites can be highly variable, making them ideal for DNA fingerprinting. DNA is also stained or made visable by use of a radioactive probe. = = = = What is the Process? = = 1. The process begins with a cell or blood sample where DNA is extracted. = = 2. The DNA is cut into fragments using a restriction enzyme.then the fragments are the seperated into bands. = = 3. The DNA band pattern is transferred to a nylon membrane. = = 4. A DNA probe binds to specific DNA sequences on the nylon membrane. = = 5. The excess probe materiall is wased away leaving the unique DNA band pattern. = = 6. The radioactive DNA pattern is transfed to x-ray film by direct exsposer. When developed a DNA fingerprint is what is left. =

= = = What is DNA **Fingerprinting**? = = DNA fingerprinting is a way to identify a specific individual. = = = = = = What is it used for? = = DNA fingerprinting is useful for determining a person in a crime, for paternity and maternity tests, for finding missing children, or to identify a body. DNA fingerprinting requires a sample of blood, skin, tissue, etc. =

= = = How do they use it? = = When a crime is commited, detectives go to the scene to try and identify the suspect and/or the victim. They can look on surrounding objects for fingerprints to identify the person. Also, they can take blood samples to help them determine who the person or persons are. = = = ==

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